Figure 3
From: p53 dynamics orchestrates with binding affinity to target genes for cell fate decision
MDM2 repression facilitates cell apoptosis without the change of E∫p53. (a) Regulatory network of p53 in response to chemotherapy. (b) Apoptotic rate of cells treated with Dox with or without MDM2 silencing for 72 h. Data are represented as mean±S.E.M.; **P<0.01; ***P<0.001. (c and d) Lifespan and E∫p53 level of cells treated with 0.1 μM Dox combined with or without MDM2 silencing. Data are represented as mean±S.E.M.; ***P<0.001. (e) Representative single-cell traces of MCF7-p53-Venus cells treated with 0.1 μM Dox combined with Nutlin-3 (10 μM) added immediately or at 6 and 16 h, following Dox treatment, respectively. ‘N’ with an arrow in the figure indicates the time when Nutlin-3 (N) was added. (f) The rate of cells exhibiting terminal pulse (terminal rate) under different treatments within 96 h (n=293 in total). (g–i) Lifespan, ∫p53 and E∫p53 levels of apoptotic cells treated with 0.1 μM Dox and co-administered with Nutlin-3 added to medium at the indicated time points (n=175 in total). Error bars represent S.E.M.; *P<0.05; ***P<0.001. (j) Single cell traces of E∫p53 in response to 0.1 μM Dox treatment co-administered with Nutlin-3. Green, surviving cells (n=17); red, apoptotic cells (n=175). NS, not significant
