Figure 5 | Cell Death & Disease

Figure 5

From: ANP promotes proliferation and inhibits apoptosis of ovarian granulosa cells by NPRA/PGRMC1/EGFR complex and improves ovary functions of PCOS rats

Figure 5

NPRA/PGRMC1/EGFR complex promoted the proliferation of ovarian granulosa cells through the MAPK/ERK signaling pathway. KGN cells were treated with RU486, or RU486 and ANP, respectively. (a) Immunoprecipitation (IP): anti-NPRA pulled down proteins. Immune blot (IB): levels of PGRMC1 and EGFR were detected by western blot. (b) IP: anti-PGRMC1 pulled down proteins. IB: levels of NPRA and EGFR were detected by western blot. (c) KGN cells were treated with RU486, RU486 and ANP, or the inhibitor of MAPK/ERK (PD58095). Phosphorylation of EGFR (p-EGFR) and ERK1/2 (p-ERK1/2) were detected by western blot. (d) Western blot analysis of PCNA expression in KGN cells. Statistical analysis was shown. Significance was indicated by *P<0.05, **P<0.01, ***P<0.001

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