Figure 2

Activation of CB₂R promotes gonocyte meiotic entry. (a) Representative immunofluorescence images showing SCP3 (green) organization on nuclear spreads at the stages of preleptotene, early leptotene and leptotene cells of meiotic prophase I. (b) Histogram representing the percentage of nuclei with meiotic SCP3 staining in male germ cells from E13.5 gonads after 48 h of culture in the absence or presence of JWH133 alone or in combination with AM630. (c) Percentage of preleptotene, early leptotene and leptotene nuclei in cultures of E13.5 male germ cells treated and untreated for 48 h with JWH133 alone or in combination with AM630. (d) Histogram representing the percentage of nuclei with meiotic SCP3 staining in E15.5 male germ cells treated and untreated for 48 h with JWH133 alone or in combination with AM630. (e) Percentage of preleptotene, early leptotene and leptotene nuclei in cultures of E15.5 male germ cells treated and untreated for 48 h with JWH133 alone or in combination with AM630. (f and g) Real-time PCR of meiotic genes Stra8 and Nanos2 in E13.5 male germ cells treated or not for 48 h with JWH133. Data were collected from at least three different experiments, using a minimum of 10 embryos for each one. *P<0.05 and **P<0.01