Figure 3
From: Overactive type 2 cannabinoid receptor induces meiosis in fetal gonads and impairs ovarian reserve
Activation of CB2R promotes fetal oocyte meiotic entry at E13.5. (a) Representative immunofluorescence images showing SCP3 (green) organization on nuclear spreads at the stages of preleptotene, early leptotene, leptotene, zygotene and pachytene cells of meiotic prophase I. (b) Histogram representing the percentage of nuclei with meiotic SCP3 staining in female germ cells from E13.5 gonads treated or not with JWH133 alone or in combination with AM630 for 48 h. (c) Percentage of meiotic nuclei at different stages of prophase I in female germ cells from E13.5 gonads treated or not for 48 h with JWH133 alone or in combination with AM630. (d) Real-time PCR of meiotic genes Dmc1, Spo11, c-Kit, SCP1, SCP3, Stra8 and Nanos2 in female germ cells from E13.5 gonads treated or not for 48 h with JWH133. Data were collected from at least three different experiments, using a minimum of 10 embryos for each one. *P<0.05 and **P<0.01
