Figure 4
From: Overactive type 2 cannabinoid receptor induces meiosis in fetal gonads and impairs ovarian reserve
Activation of CB2R promotes fetal oocyte meiotic progression at E15.5. (a) Representative immunofluorescence images showing SCP3 (green) organization on nuclear spreads at the stages of leptotene, zygotene, pachytene, diplotene and metaphase-like cells of meiotic prophase I. (b) Histogram representing the percentage of nuclei with meiotic SCP3 staining in female germ cells from E15.5 gonads treated or not with JWH133 for 48 h. (c) Percentage of meiotic nuclei at different stages of prophase I of female germ cells from E15.5 gonads treated or not for 48 h with JWH133. Data were collected from at least three different experiments, using a minimum of 10 embryos for each one. NS, not significant. *P<0.05
