Figure 5
From: Overactive type 2 cannabinoid receptor induces meiosis in fetal gonads and impairs ovarian reserve
Activation of CB2R increases γ-H2AX foci in fetal oocytes. (a) Representative immunofluorescence images showing staining of SCP3 (red) and γ-H2AX (green) on pachytene, diplotene and metaphase-like cells from E15.5 ovary. (b) Percentage of double-positive SCP3 and γ-H2AX oocytes at the stages of pachytene, diplotene and metaphase-like from E15.5 ovary, treated or not with JWH133 for 48 h. Data were collected from at least three different experiments, using a minimum of 10 embryos for each one
