Figure 6
From: Overactive type 2 cannabinoid receptor induces meiosis in fetal gonads and impairs ovarian reserve
Activation of CB2R increases the number of terminal deoxinucleotidyl transferase-mediated dUTP-fluorescein nick end labeling (TUNEL)-positive oocytes. (a) Representative image of spreads nuclei of E15.5 female germ cells stained for SCP3 (red) and TUNEL (green). An SCP3-positive cell at the pachytene stage, a TUNEL-positive cell with disassembled pattern of synaptonemal complex (indicated by white arrow) and TUNEL-negative cells are shown. (b) Percentage of SCP3 and TUNEL double positive in female germ cells from E15.5 gonads treated or not with JWH133 for 48 h. Data were collected from at least three different experiments, using a minimum of 10 embryos for each one. *P<0.05
