Figure 7
From: Overactive type 2 cannabinoid receptor induces meiosis in fetal gonads and impairs ovarian reserve
In vivo treatment of pregnant mice with JWH133 affect ovarian reserve of female pups. (a) Morphological staining with H&E of 1 dpn ovaries in utero exposed to JWH133 shows a reduction in follicle number and size as shown at higher magnification on the right (Pr.: primordial follicles). (b) The histograms show a significant reduction in primordial and primary follicle number in the ovary of F1 offspring in utero exposed to JWH133 with respect to vehicle-exposed offspring. (c) A significant reduction in the diameter of primordial follicles was detected in the ovaries of F1 from JWH133-treated pregnant mice with respect to F1 primordial follicles from control pregnant mice. (d) Mating rate of F1 female from JWH133-treated pregnant mice was unchanged with respect to mating rate of F1 control mice. (e) Litter size of F2 from JWH133 F1 female was significantly reduced with respect to that F1 control mice. Data were collected using a minimum of three 1 dpn female pups. NS, not significant. *P<0.05
