Figure 2
A time-dependent Rel protein expression pattern was induced by Aβ1-40 both in vivo and in vitro. (a) qRT-PCR assays of Rel gene expression in RPE–choroid complexes. Western blots showing time-dependent expression of Rel proteins in cytoplasmic (b) and nuclear extracts (c) of WT RPE–choroid complexes treated with 5 μl of 2.8 μg/μl Aβ1-40 or PBS (0, 2 and 4 days). Histone H3 was used as a quality control for the nuclear extracts. (d) Rel protein expression in mouse RPE cells in response to 5 μM Aβ1-40 at 6, 12 and 24 h after treatment. UT, untreated; WT, wild type. Histograms represent the mean and S.E.M. N=3, NS=nonsignificant P-value, *P<0.05, **P<0.01, ***P<0.001 via Student’s t-tests
