Table 1 Derivation of neural stem cells

From: Current progress in the derivation and therapeutic application of neural stem cells

Species

Original tissues/cells

Treatment

Duration to derive NSCs

Refs

Isolation from primary tissues

 Mouse

Striatum

EGF

1 week

1

 Mouse

Thoracic spinal cord

EGF, bFGF

1 week

3

 Mouse

Dentate gyrus, SVZ

EGF, bFGF

1–3 weeks

4,10,11

 Mouse

Periventricular region

EGF, bFGF, heparin

2 weeks

5

 Human and rat

Periventricular region

EGF, bFGF, heparin

2–3 weeks

6

 Mouse

Olfactory bulb

EGF, bFGF

1–2 weeks

7

 Human

Olfactory bulb

EGF, bFGF

1–2 weeks

8

 Mouse

Postnatal cerebellum

EGF, bFGF

2 weeks

9

Differentiation from pluripotent stem cells

 Human

ESCs

Suspension culture

3–4 weeks

13

 Human

ESCs

Adhesion co-culture with stromal cells MS-5

3 weeks

18

 Human

ESCs

Adherent monolayer culture

3–5 weeks

20

 Human

iPSCs

Suspension and adherent culture

2–5 weeks

21

Transdifferentiation from somatic cells

 Mouse

Fibroblasts

Oct4, Sox2, Klf4, c-Myc

2–3 weeks

24,25

 Mouse

Fibroblasts

Brn4/Pou3f4, Sox2, Klf4, c-Myc, E47/Tcf3

4–5 weeks

26

 Mouse

Fibroblasts

Foxg1, Sox2, Brn2

3–4 weeks

27

 Mouse and human

Fibroblasts

Sox2

2–3 weeks

28

 Human

Fibroblasts

ZFP521

3–4 weeks

29

 Primate

Fibroblasts

OCT4, SOX2, KLF4, c-MYC, SB431542, CHIR99021

2–3 weeks

30

 Human

Fibroblasts

OCT4, A83-01, CHIR99021, NaB, LPA, Rolipram, SP600125

4 weeks

31

 Mouse

Sertoli cells

Ascl1, Ngn2, Hes1, Id1, Pax6, Brn2, Sox2, c-Myc, Klf4

4–5 weeks

32

 Mouse

Liver cells and B cells

Brn2, Hes1, Hes3, Klf4, c-Myc, Notch1, PLAGL1, Rfx4

4–5 weeks

33

 Human

Urine cells

SOX2, KLF4, c-MYC, SV40LT, miR302-367, A83-01, PD0325901, CHIR99021, Thiazovivin, DMH1

4–5 weeks

34

 Human

Astrocytes

OCT4, SOX2, NANOG

2–3 weeks

35

 Human

Cord blood CD34+ cells

OCT4, CHIR99021

2–3 weeks

36

 Mouse and human

Fibroblasts, urine cells

VPA, CHIR99021, Repsox

3 weeks

37

 Mouse

Fibroblasts

CHIR99021, LDN193189, A83-01, Hh-Ag1.5, Vc, SMER28, RG108, Parnate

2 weeks

38

 Mouse

Fibroblasts

VPA, Forskolin, Tranylcypromine, CHIR99021, Repsox, SB431542, Dorsomorphin

2 weeks

39

 Mouse

Fibroblasts

VPA, A83-01, Purmorphamine, Vc, NaB, Thiazovivin

2 weeks

40

 Mouse and human

MSCs

bFGF, EGF

1–2 weeks

42,43

 Mouse

Fibroblasts

bFGF, EGF, heparin, LIF

3–4 weeks

45

Mouse

Fibroblasts

3D sphere culture

NA

46

  1. Abbreviations: bFGF, basic fibroblast growth factor; 3D, three dimension; EGF, epidermal growth factor; ESCs, embryonic stem cells; iPSCs, induced pluripotent stem cells; LIF, leukemia inhibitory factor; LPA, lysophosphatidic acid; MSCs, mesenchymal stem cells; NA, not available; NaB, sodium butyrate; SVZ, subventricular zone; Vc, ascorbic acid; VPA, valproic acid
Back to article page