Figure 3
From: Impairment of PTX3 expression in osteoblasts: a key element for osteoporosis
Sclerostin and OPG expression. Immunohistochemistry results were evaluated by a semiquantitative approach assigning a score from 0 to 3 according to the number of positive osteoblasts for OPG and osteocytes for sclerostin (Table 2). (a) OP patient showed numerous sclerostin-positive osteocytes (square). (b) Trabeculae of a OA patient characterized by both positive (arrow) and negative osteocytes. (c) Rare sclerostin-positive osteocyte cells in CTRL patients. (d) OP patient with low expression of OPG. (e) OPG-positive cells localized on the trabecular surface in a OA patient. (f) Numerous OPG positive cells both on the trabecular surface and in bone marrow (arrows) in CTRL patients. Scale bar represents 40 μm in all images. (g) Graph shows immunohistochemical results of sclerostin (OP versus OA P<0.0001; OP versus CTRL P<0.0001; OA versus CTRL P=0.0242). (h) Graph shows immunohistochemical results of OPG (OP versus OA P=0.355; OP versus CTRL P<0.0001; OA versus CTRL P<0.0001). (OP n=25, OA n=25, CTRL n=15)
