Figure 1 | Cell Death & Disease

Figure 1

From: iASPP facilitates tumor growth by promoting mTOR-dependent autophagy in human non-small-cell lung cancer

Figure 1

Ectopic expression of iASPP induces autophagy in H1975 cell. (a) Endogenous levels for LC3 (green fluorescence) was visualized on confocal microscope by immunofluorescenc staining. (b) LC3 dots in control or iASPP-overexpressing cells treated with autophagy inducer as in (a) were counted. Data represent mean±S.E.M. of at least 100 cells scored (*P<0.05, **P<0.001). (c) Conversions of LC3-I to LC3-II in cells treated as in (a) were determined by western blotting. GAPDH was a loading control. (d) The ratio of endogenous LC3-II to LC3-I protein level treated as in c was counted. The value in the iASPP-overexpressing cells without any treatment was normalized as 1

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