Figure 1
From: Differential regulation of spermatogenic process by Lkb1 isoforms in mouse testis
Expression of Lkb1L and Lkb1S in different spermatogenic cell types. Spermatogenic cells at different developmental stages (SP – spermatocytes, RS – round spermatids and ES – elongated spermatids) were sorted from pooled adult testes via STA-PUT velocity sedimentation. Mouse SSCs were enriched from P8 testes and cultured in vitro for at least five passages. All experiments were performed with at least three replicates. (a–c) The purity of these cells was confirmed by RT-PCR using markers: (a) Plzf for SSC, (b) Sycp2 for SP, (c) Ups26 for CS and ES. (d) RT-PCR for Lkb1L and Lkb1Stranscripts in each spermatogenic cell type. (e) Western blot analysis of Lkb1L and Lkb1Sin adult testis and SSCs
