Figure 3
From: Differential regulation of spermatogenic process by Lkb1 isoforms in mouse testis
Abnormal acrosomal biogenesis in Lkb1 cKO mice. Testes from both control and cKO mice (n=3) were collected at 6 weeks of age for tubule analysis. (a) Representative images of control and comparable cKO testis sections are shown at developmental stages I–VIII, IX, X and XII. Asterisks mark giant and round cells. Scale bars=50 μm. (b) Higher magnification of acrosomes stained by fluorescently tagged peanut agglutinin at different stages of spermatogenesis. Scale bars=25 μm. Asterisks, round spermatid with multiple pre-acrosomal vesicles. (c) Ultrastructural analysis of acrosome formation in control and Lkb1 cKO spermatids. Arrowheads, acrosomal granules or acrosomes. Black arrows, closer leaflets of nuclear envelope. White arrows, developing axonemes or centriole-like organelle. Black asterisks, spermatid nuclei. Scale bar=1 μm. (d and e) RT–PCR analysis for spermiogenesis-related key regulator genes in adult (d) and P24 (e) control and cKO mice. Data are presented as means ± S.E.M. of at least three replicates. *P<0.05 and **P<0.01, compared with control mice. The level of mRNA expression in control mice was given an arbitrary value of 1
