Figure 7

Increased mTORC1 activity in germ cells from Lkb1 newborn cKO testis. (a) Western blot of testis proteins at different postnatal ages using specific antibodies against p-S6K1 (T389), p-rpS6 (S235/6), rpS6 and β-tubulin. rpS6, and β-tubulin were used as internal controls. (b) Expression of PI3K-, mTOR- and SPC-related markers by western blot in P8 testes from control and Lkb1 cKO mice. (c) Immunohistochemistry for VASA, GATA4, p-rpS6 in P8 control testis. (d) Immunohistochemistry for Lin28a and p-rpS6 in P8 control testis. Serial sections were used in order to compare cellular localizations of these markers in the same tubule. Asterisks, different distribution of Lin28a and p-rpS6 in the same tubule resulting in strong positive for Lin28a and weak staining for p-rpS6. Arrows, cells expressing both Lin28a and p-rpS6. (e) Immunohistochemistry for Plzf and p-rpS6 in control and cKO mice. (f) Mean number of Plzf- or p-rpS6-positive cells in each tubule (positive cells/total tubules). The data were shown as mean±S.E.M. of at least three replicates. *P<0.05. Scale bars=50 μm