Figure 10

circLMO7 inhibits apoptosis in bovine primary myocytes. (a, b) We transfected bovine primary myocytes with pcDNA-circLMO7 and/or miR-378a-3p mimic and determined apoptosis by Annexin V-FITC/PI binding followed by flow cytometry. (c) mRNA of apoptosis marker genes (BCL-2, BAX, caspase9) was detected using qPCR. (d) Proteins of apoptosis marker genes were detected using western blots. Data are presented as means±S.E.M. for three individuals. *P<0.05