Figure 7 | Cell Death & Disease

Figure 7

From: Circular RNA profiling reveals an abundant circLMO7 that regulates myoblasts differentiation and survival by sponging miR-378a-3p

Figure 7

Expression analysis of circLMO7 using qPCR. (a) Expression levels of circLMO7 in different tissues of embryonic cattle. (b) Visualization of the efficiency of circLMO7 overexpression vector pcDNA-circLMO7. (c) Effect of circLMO7 on the abundance of miR-378a-3p. (d) RNAhybrid predicted a miR-378a-3p binding site and provided ΔG values for its interaction with circLMO7. (e) Our miR-378a-3p overexpression vector was co-transfected with psiCHECK2-circLMO7W or psiCHECK2-circLMO7M into bovine primary myocytes. Renilla luciferase activity was normalized to Firefly luciferase activity. (f) mRNA expression of HDAC4 in primary bovine myocytes transfected with circLMO7 and/or miR-378a-3p mimic for 24 h was detected using qPCR. Data are shown as means±S.E.M. for three individuals. *P<0.05

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