Figure 9

circLMO7 promotes the proliferation of bovine primary myocytes. (a, b) Bovine primary myocytes were transfected with pcDNA-circLMO7 and/or miR-378a-3p mimic, and cell phases were analyzed by flow cytometry. (c) Cell proliferation was assessed using the cell counting kit-8 (CCK-8) assay. (d) mRNA of the proliferation marker CyclinD1 was quantified using qPCR. (e) Cell proliferation indices were assessed after treatment with 5-Ethynyl-2′-deoxyuridine (EdU). The scale bar represents 200 μm. Data are presented as means±S.E.M. for three individuals. *P<0.05