Figure 1 | Cell Death & Disease

Figure 1

From: Glucocorticoid mediates prenatal caffeine exposure-induced endochondral ossification retardation and its molecular mechanism in female fetal rats

Figure 1

Effects of PCE on fetal femurs histology, variations of proliferation and differentiation. (a) IUGR rate. (b) Length of femur (mm). (c) H&E staining of fetal femur. (d) Von Kossa staining of fetal femur. (e) Length of primary ossification center (POC) (mm). (f) POC/femur (%). (g) Length of hypertrophic chondrocytes zones (HZ) (mm). (h) HZ/femur (%). (i) Length of pre-hypertrophic chondrocytes zones (PHZ) (mm). (j) PH/femur (%). (k) Length of proliferative chondrocytes zones (PZ) (mm). (l) PZ/femur (%). (m) Immunostaining of Ki67. (n) Immunostaining of p57. (o) Quantification of Ki67 (positive rate). (p) Quantification of P57 (positive rate). n=5 per group obtained from different litters. Three random fields/section for quantitative. Data are shown as the mean±S.D. *P<0.05, **P<0.01 versus control (ANOVA)

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