Figure 2 | Cell Death & Disease

Figure 2

From: Glucocorticoid mediates prenatal caffeine exposure-induced endochondral ossification retardation and its molecular mechanism in female fetal rats

Figure 2

Effects of prenatal caffeine expose (PCE) on terminal differentiation of hypertrophic chondrocytes in fetal long bone. (a) Immunostaining of Runt-related transcription factor 2 (Runx2) in hypertrophic chondrocytes. (b) In situ hybridization (ISH) of collagen type X (Col-X) in hypertrophic chondrocytes. (c) Fluorescent TdT-mediated dUTP nick-end labeling (TUNEL) staining in hypertrophic chondrocytes. (d) Quantification of Runx2 (positive rate). (e) Quantification of Col-X ISH (fluorescence intensity). (f) Quantification of fluorescent TUNEL (positive rate). n=5 per group obtained from different litters. Three random fields/section for quantitative. Data are shown as the mean±S.D. *P<0.05, **P<0.01 versus control (ANOVA)

Back to article page