Figure 4 | Cell Death & Disease

Figure 4

From: Glucocorticoid mediates prenatal caffeine exposure-induced endochondral ossification retardation and its molecular mechanism in female fetal rats

Figure 4

Effects of corticosterone (250–1250 nM) with/without siRNA (Mig-6, EGFR) for 48 h on rats primary chondrocytes terminal differentiation and apoptosis. (a and b) mRNA expression of mitogen-inducible gene 6 (Mig-6) and EGFR after corticosterone and Mig-6 siRNA treatment, (c) Protein expression of Mig-6, EGFR, phosphorylated EGFR (P-EGFR), c-Jun N-terminal kinase (JNK) and Phosphorylated JNK (P-JNK) detected by western blotting after corticosterone and Mig-6 siRNA treatment. (d–h) Quantification of Mig-6, EGFR, P-EGFR, JNK and P-JNK (relative grayscale). (i–k) mRNA expression of runt-related transcription factor 2 (Runx2), collagen type X (Col-X) and matrix metalloproteinases-13 (MMP-13) after corticosterone and Mig-6 siRNA treatment. (l and m) Apoptotic analysis detected by Annexin V/PI after corticosterone and Mig-6 siRNA treatment. (n) Protein expression of EGFR, JNK and P-JNK detected by western blotting after EGFR siRNA treatment. (o) Quantification of Mig-6, EGFR, P-EGFR, JNK and P-JNK (Relative grayscale). (p) mRNA expression of Runx2, Col-X and MMP-13 after EGFR siRNA treatment. (q) Apoptotic analysis detected by Annexin V/PI after EGFR siRNA treatment. Data are shown as the mean±S.D. of results from three experiments. *P<0.05, **P<0.01 versus control; #P<0.05, ##P<0.01 versus CORT treatment group. (A-M, ANOVA; O-R, t test)

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