Figure 5

Effects of caffeine (0.1–100 μM) on primary rat chondrocytes terminal differentiation and apoptosis. (a) mRNA expression of mRNA expression of runt-related transcription factor 2 (Runx2), collagen type X (Col-X) and matrix metalloproteinases-13 (MMP-13). (b and c) Apoptotic analysis by Annexin V/PI. Data are shown as the mean±S.D. of results from three experiments. *P<0.05, **P<0.01 (ANOVA)