Table 1 Primer used for real-time quantitative polymerase chain reaction

From: Glucocorticoid mediates prenatal caffeine exposure-induced endochondral ossification retardation and its molecular mechanism in female fetal rats

Genes

Forward primer

Reverse primer

Mig-6

CCTACAATCTGAACTCCCCTG

AGCTTGACTTTGGAGATGGAC

EGFR

ACCAGCAGGACTTCTTTCCCA

TAAACTCACTGCTTGGCGGTG

Runx2

GCCACCTTCACTTACACCCC

CGCTGACGAAGTACCATAGTAGAG

MMP-13

TGACCTGGGATTTCCAAAAGAG

GTCTTCCCCGTGTCCTCAAA

Col-X

GCGCATCATGAATCTCGTTT

GGGTTGTGTGAACCATGGAG

GAPDH

GCAAGTTCAACGGCACAG

GCCAGTAGACTCCACGACA

  1. Col-X, collagen type X; EGFR, epidermal growth factor receptor; GAPDH, glyceraldehydes 3-phosphatedehydrogenase; Mig-6, mitogen-inducible gene 6; MMP-13, matrix metallopeptidase 13; Runx2, runt-related transcription factor 2.
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