Figure 2
From: CITED2 affects leukemic cell survival by interfering with p53 activation
Loss of CITED2 triggers apoptosis in leukemic cells. (a) The leukemic cell lines MOLM-13 and NB4 were transduced with a GFP-expressing control- or shCITED2 construct. The number of GFP+ cells over time is shown. Error bars indicate s.d. of three individual experiments; *P<0.05. (b) Annexin V staining of NB4 and MOLM-13 cells 4–5 days after transduction with shCITED2. FACS plots of an individual experiment (left panels) and average percentage of Annexin V-positive cells from multiple experiments (right panels) is shown. Error bars indicate s.d. (n=6 MOLM-13, n=4 NB4). ***P<0.001, *P<0.05. (c) Cell-cycle analysis of control- and shCITED2-transduced MOLM-13 (left panel) and NB4 cells (right panel)
