Figure 2
From: PLOD2 regulated by transcription factor FOXA1 promotes metastasis in NSCLC
Epidermal growth factor receptor (EGFR) inhibitor decreased metastasis possibly via PLOD2, in vitro and in vivo. (a) WZ4002 inhibited the expression of PLOD2 in the NCI-H1975 cell line. (b) Knockdown of EGFR decreased the expression of PLOD2 by small interfering RNA (siRNA) in the NCI-H1975 cell line. (c) The EGFR ligand (tumor growth factor-α (TGF-α)) promoted the expression of PLOD2 in the A549 cell line. (d) The protocol of the NSCLC metastasis model generated by orthotopic implantation. (e) The number of metastasis nodes was decreased by WZ4002. (f) Representative micro-positron emission tomography (micro-PET) image of mice 4 weeks after implantation. The color scale is indicated. (g) Further confirmation of the metastasis nodes on the chests and lungs by hematoxylin and eosin (HE) staining. (h and i) The expression of P-EGFR and PLOD2 was consistently decreased by WZ4002 in the tumor tissues. (j and k) Fibrillar collagen formation and collagen deposition detected by picrosirius red and Masson’s trichrome staining in the tumor tissues.The degree of fibrillar organization was evaluated by a pathologist. The score (from 0 to 5) indicates the degree of fibrillar organization. Higher score suggested greater fibrillar organization. The data were represented as the mean±S.D. of three independent experiments. The P-values <0.05 were considered statistically significant for all tests
