Figure 4
From: PLOD2 regulated by transcription factor FOXA1 promotes metastasis in NSCLC
Knockdown of PLOD2 decreased the metastasis in vivo. (a) The protocol of the development of the NSCLC metastasis model based on orthotopic implantation. (b) Knockdown of PLOD2 decreased metastasis in vivo. (c) Representative micro-positron emission tomography (micro-PET) image of mouse, 4 weeks after implantation. The color scale is indicated. (d) Knockdown of PLOD2 improved the life quality of the NSCLC metastasis model. (e) The metastasis nodes were outside and inside the chest based on gross examination. The metastasis nodes on the chests and the lungs were further confirmed by hematoxylin and eosin (HE) staining. (f and g) Fibrillar collagen formation and collagen deposition was detected by picrosirius red and Masson’s trichrome staining in the tumor tissues, when PLOD2 was knocked down by short hairpin RNA (shRNA). The degree of fibrillar organization was evaluated by a pathologist. The score (from 0 to 5) indicates the degree of fibrillar organization. Higher score suggested greater fibrillar organization. The data were represented as the mean±S.D. of three independent experiments. The P-values <0.05 were considered statistically significant for all tests
