Figure 6

FOXA1 might be the transcription factors of PLOD2. (a) FOXA1 was selected as the potential transcription factor of PLOD2 based on a series of databases. First, the promoter sequence of PLOD2 was obtained via the Ensemble project and Mapviewer. Then, the 769 potential transcription factors were predicted using the Genomatix database. Finally, the five most potential transcription families were screened out based on the prediction score (the matrix sim). Based on a Kaplan–Meier (KM) plotter analysis, the Oncomine database, JASPAR database and TCGA Research Network, FOXA1 may be a potential transcription factor. (b and c) The effect of Gefinitib and tumor growth factor-α (TGF-α) on the transcription of PLOD2 was evaluated by luciferase reporter assays. (d) The expression of FOXA1 was higher in adenocarcinoma tissues than in normal tissues according to the Oncomine database. (e) KM analysis of relapse-free survival in lung cancer patients was from KM plotter. PLOD2 in lung adenocarcinoma patients at stage I (n=370). (f) Correlation analysis of FOXA1 and PLOD2 in lung adenocarcinoma patients based on the TCGA Research Network. (g and h). Knockdown of FOXA1 decreased the expression of PLOD2 by small interfering RNA (siRNA) in the NCI-H1975 cell line. (i and j) Knockdown of FOXA1 decreased the cell migration. The data were represented as the mean±S.D. The P-values<0.05 were considered statistically significant for all tests