Figure 7
From: PLOD2 regulated by transcription factor FOXA1 promotes metastasis in NSCLC
The phosphatidylinositol-3-kinase/AKT (PI3K/AKT) signaling pathway promoted the expression of PLOD2 via the FOXA1 transcription factors. (a) The PI3K/AKT signaling pathway inhibitor (LY294002) consistently decreased the expression of FOXA1 and PLOD2. (b and c) The PI3K/AKT signaling pathway inhibitor (LY294002) inhibited the nuclear translocation by separation of the cytoplasm and nucleus, as revealed by the immunofluorescence assay. (d) Knockdown of FOXA1 decreased the expression of PLOD2 by small interfering RNA (siRNA)in the A549 cell line. (e–g) Knockdown of FOXA1 reversed the upregulation of PLOD2 induced by tumor growth factor-α (TGF-α) in A549 cell line. (h) FOXA1 bind to three putative bind regions upstream of the transcription start site (TSS) of PLOD2 gene. PI3K/AKT signaling pathway inhibitor (LY294002) inhibited the bind regions of PLOD2 gene. Precipitated DNA fragments in ChIP assays were examined by quantitative reverse transcription-PCR (qRT-PCR). Immunogobulin G (IgG) was used as a negative control. (i) Schematic representation of the PLOD2 locus. FOXA1-binding sites upstream of the TSS of PLOD2 were predicted by ChIP assay. The P-values <0.05 were considered statistically significant for all tests
