Figure 5

Deletion of Ccnb1 in germ cells promoted their differentiation and Lin28a/let-7 axis involved in this process. (a) Real-time PCR analysis of Plzf, c-Kit, Lin28a and Gdnf expression in 2 dpn control and Mvh-cKO mice testis. (b) Real-time PCR analysis of Plzf, c-Kit, Lin28a and Gdnf expression in 3 dpn control and Mvh-cKO mice testis. (c) Real-time PCR analysis of Plzf, c-Kit, Lin28a and Gdnf expression in 7 dpn control and Mvh-cKO mice testis. (d) Real-time PCR analysis of Plzf, c-Kit, Lin28a and Gdnf expression in 90 dpn control and Stra8-cKO mice testis. (e) Real-time PCR analysis of let-7 family miRNAs expression in 90 dpn Control and Stra8-cKO mice testis. In panels (a–e), ≥3 samples were used in each group in qPCR