Figure 7
MuV-induced CXCL10 production and germ cell apoptosis in the testis. MuV (1 × 107 PFU) in 10 μl of PBS was injected into the testis of 5-week-old WT and CXCL10−/− mice. The contralateral testis that was injected with an equal volume of PBS alone served as Ctrl. (a) MuV detection. MuV-NP in testicular lysates was detected by western blot analysis at 24 h after MuV injection. (b) CXCL10 level. The testis was lysed by grinding in PBS at 24 h after MuV injection. CXCL10 levels in the lysates were measured using ELISA. (c) Apoptosis of male germ cells. The testis of WT (left panels) and CXCL10−/− (right panels) mice were injected with PBS or MuV. After 24 h, apoptotic germ cells in paraffin sections were detected using co-staining of TUNEL and IF with antibodies to MVH. Insets in upper right corners represent the higher resolution for dotted box areas (lower panels). (d) A time-dependent germ cell apoptosis. The testes of WT and CXCL10−/− mice were injected with MuV for the indicated durations. Apoptotic germ cells were quantitatively analyzed based on the co-staining of TUNEL and IF. Apoptotic germ cell numbers per tubular section were presented. One hundred tubules per testis were counted. Images are the representatives of three mice. Scale bar=40 μm. Data represent the means±S.E.M. of three mice. *P<0.05 and **P<0.01
