Figure 8
MuV-mediated impairment of spermatogenesis. (a) Histological analysis. MuV (1 × 107 PFU) in 10 μl of PBS was injected into the testis of 5-week-old C57BL/J6 mice (lower panels). Equal volume of PBS alone was injected into the contralateral testis for the control (upper panels). Histological analysis was performed on the paraffin sections after hematoxylin and eosin (HE) staining. Asterisk indicate seminiferous tubules (ST) without elongated spermatids. (b) Quantification of spermatogenesis impairment. C57BL/J6 mice were treated as described in a. The ratio of the ST with elongated spermatids in lumen was determined based on histological analysis. A total of 200 tubules in three sections were spontaneously counted for spermatogenesis examination. (c) Spermatogenesis impairment in TNF-α−/− and CXCL10−/− mice. 5-week-old TNF-α−/− and CXCL10−/−, as well as respective control mice, were treated as described in (a). The ratio of ST with elongated spermatids in lumen was determined at 2 weeks after MuV injection. Images are the representatives of three mice. Scale bar=100 μm. Data represent the means±S.E.M. of three mice. **P<0.01
