Figure 3
From: Multiple mechanisms mediating carbon monoxide inhibition of the voltage-gated K+ channel Kv1.5
CO stimulates NO formation and nitrosylation of hKv1.5. (a) Mean (±S.E.M.) fluorescence recorded in cells loaded with the NO indicator, DAF-2. Fluorescence was measured in untreated (control) cells (open circles, n=5) or cells pretreated with L-NAME (1 mM; 1 h, 37 °C; n=5). (b) Example images at 0 and 10 min after commencement of CORM-2 application, as indicated, in untreated and L-NAME-treated cells. Scale bar (20 μm) applies to all panels. (c) Upper; western blotting showing similar levels of hKv1.5 in cell lystates treated with CORM-2 or iCORM (30 μM, 1 h). Lower, detection of nitrosylation via the biotin-switch assay. Note: nitrosylation of hKv1.5 was only detected in samples treated with CORM-2
