Figure 2

miR-1 suppresses cell proliferation. (a) Representative fluorescence images of colorectal cancer cells, HCT-116, HT-29 and CaCO2, infected lentivirus as indicated, and (b) RT-PCR analysis was performed to verify miR-1 expression level, Data are expressed as mean±s.d., n=3 in each group. *P<0.05; **P<0.01; ***P<0.001 between groups using one sample t-test. (c–e) the time course of miR-1 on cell proliferation was evaluated by CCK-8 assay in HCT-116, CaCO2 and HT-29 cells, respectively. Data were presented as mean (±s.d.). n=3 in each group, *P<0.05; **P<0.01; ***P<0.001 versus control using unpaired Student’s t-test