Figure 3
miR-1 inhibits tumor glycolysis and key enzymes expression. (a, d, g) the effect of miR-1 on the concentrate of glucose consumption and lactate production in colorectal cancer cells was measured as described in Material and Methods and difference between group analyzed by using unpaired Student’s t-test, n=3, **P<0.01; ***P<0.001 versus miRNA-NC. (b, e, h) Protein levels of metabolic enzymes in the Warburg effect were determined by western blot in HT-29, HCT-116 and CaCO2 cells overexpressing miRNA-NC, miR-1mimics and miR-1 inhibitor. α-tubulin served as loading control, for each marker band, values were normalized to internal control and the density of control was set at 1.0. (c, f, i) Indicated gene mRNA levels was measured by Real-time PCR in HT-29, HCT-116 and CaCO2 cells expressing NTC or miR-1 mimics. Data were presented as mean (±s.d.). n=3 in each group. ***P<0.001 (One sample t-test)
