Figure 5
miR-1 is required for Smad3 transactivity and ectopic expression of Smad3 reversed the phenomenon caused by miR-1. (a) ChIP analysis of binding of Smad3 protein to indicated genes promoter in HT-29 cells transfected with miR-1 mimics or miR-1 inhibitor. The PCR products were subjected to agarose gel electrophoresis, for each marker band, values were normalized to internal control and the density of control was set at 1.0. (b, d) glucose uptake and lactate production in supernatant were measured in indicated group in HT-29 cells. Data were presented as mean±s.d., Student’s t-test, **P<0.01, ***P<0.001, n=3; (c) cell proliferation was detected using CCK-8 assay in HT-29 cells after 24-hour transfection as indicated group, Data were expressed as mean±s.d., n=5, **P<0.01, Student’s t-test
