Figure 7

Effects of XIAP-siRNA on XIAP mRNA and protein expression in AR42J cells. The transfection efficiency of XIAP-siRNA-3 in AR42J cells were detected using light microscopy and fluorescent microscopy (a).The mRNA expression of XIAP was measured by real-time RT-PCR analysis (b). The protein expression of XIAP was measured by western blot (c), and quantification of XIAP protein expression was shown (d). Data are expressed as the percentage of the untreated control. Results are expressed as means with the S.E.M. of three separate experiments with statistical significance at *P<0.05 when comparison was made between XIAP-siRNA- and NT-siRNA-treated cells