Figure 8
Effects of XIAP-siRNA or embelin on cell apoptosis and necrosis in cerulein+LPS-induced AR42J cells. The transfected cells or embelin-pretreated cells were incubated in F-12K medium for 24 h with cerulein+LPS. Cell apoptosis was measured by flow cytometry as shown (a), apoptotic cells are presented in the right lower quadrant of the figure (Q4), dead cells in the right upper quadrant (Q2), living cells in the left lower quadrant (Q3) and cell debris in the left upper quadrant (Q1). Results of analytical data show that the apoptotic cells were significantly increased in XIAP-siRNA-treated cells, as compared with NT-siRNA-treated cells. There was also a marked increase of apoptotic cells in embelin-pretreated cells as compared with untransfected cells. Cell necrosis was measured by the percentage of total cellular LDH released into the extracellular medium. Results demonstrated a marked reduction in LDH release (b) in XIAP-siRNA-treated cells or embelin-pretreated cells, as compared with NT-siRNA-treated cells or untransfected cells, respectively. Results are expressed as means with the S.E.M. of three separate experiments with statistical significance at *P<0.05
