Figure 5 | Cell Death & Disease

Figure 5

From: Mechanical compression induces VEGFA overexpression in breast cancer via DNMT3A-dependent miR-9 downregulation

Figure 5

Compression-induced miR-9 downregulation was associated with VEGFA expression. (a) Luciferase reporter gene assay using the LAMC2, ITGA6, ITGB4, and EIF4E 3'-UTRs. (b) mRNA expression and (c) protein expression of LAMC2, ITGA6, ITGB4, EIF4E, and VEGFA in the CAF1, MDA-MB-231, and BT-474 transfected with anti-miR-9. For the negative control, a control miR was transfected. Data are represented as mean±S.D. Statistical significance was determined using a control- versus miR-9- or anti-miR-9-transfected-sample t-test. *, **, and *** represent P-values of 0.01–0.05, 0.001–0.01, and<0.001, respectively

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