Figure 6 | Cell Death & Disease

Figure 6

From: Mechanical compression induces VEGFA overexpression in breast cancer via DNMT3A-dependent miR-9 downregulation

Figure 6

Compression-induced expression of miR-9 target genes and VEGFA was suppressed by methylation inhibition and decompression. The effect of methylation inhibition and decompression on (a) mRNA expression and (b) protein expression of miR-9 target genes and VEGFA in CAF, MDA-MB-231, and BT-474. (c) miR-9 dependent downregulation of LAMC2, ITGA6, EIF4E, and VEGFA by methylation inhibition and decompression in CAF. CAF1 was used as a representative for CAF. The cells were compressed with the RCU of 1 for 24 h in the absence or presence of 10 μM of 5-azacytidine. For decompression, the cells were cultured for additional 24 h without compression. Data are represented as mean±S.D. ** and *** represent P-values of 0.001–0.01 and <0.001, respectively

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