Figure 7
miR-9 and its target gene expression are associated with the compressive state of the tissue. (a) Table of compression values of low- and high-compressed breast cancer tissues. Tissues were obtained from breast cancer patients after measuring compression by shear-wave elastography. (b) miR-9 expression in the low- and high-compressed tissues of breast cancer. Expression of (c) mir-9-1, (d) mir-9-2, and (e) mir-9-3 in the low- and high-compressed tissues of breast cancer. mRNA expression of (f) DNMT3A, (g) LAMC2, (h) ITGA6, (i) ITGB4, (j) EIF4E, and (k) VEGFA in the low- and high-compressed tissues of breast cancer. **, and *** represent P-values of 0.001–0.01 and <0.001, respectively. Data are represented as the mean±S.D. Statistical significance was determined using the nonparametric test of a low- versus high-compressed tissue. PLA of (l) integrin-α6β4, and (m) VEGFA. The PLA assay for ITGA6 was performed using the double recognition of rabbit anti-ITGA6 and mouse anti-ITGB4 antibodies. The PLA assay for VEGFA was performed using the single recognition of mouse anti-VEGFA antibody. Fluorescence images were quantified by measuring red-colored area using Image J. (n) A signal transduction pathway for VEGFA by compression-induced miR-9 downregulation
