Figure 5
From: Modulation of dendritic cell function by the radiation-mediated secretory protein γ-synuclein
Irradiated tumor cells decreased DC maturation and activation. (a) The secreted SNCG was detected in the conditioned media from murine mammary 4T1 tumor cells using immunoblotting. (b) The mDCs (1×106 cells) were stimulated with SNCG (1 μg/ml) or irradiated 4T1 murine breast carcinoma cells (10 Gy, 2×105 cells) for 48 h in a Transwell system. The surface maturation markers of the DCs were determined by FACS analysis. (c) The expression of IL-12 and TNF-α mRNAs isolated from DCs cocultured with SNCG or irradiated 4T1 tumor cells was investigated by real-time reverse transcription PCR and was normalized to 18S mRNA. The data represents the mean ± S.D. of three independent experiments. ***P<0.001, **P<0.01 and *P<0.05 compared with the mDC group. Significance was determined by a one-way ANOVA followed by Tukey's multiple comparison tests.
