Figure 1 | Cell Death Discovery

Figure 1

From: Caspase-independent apoptosis in infected macrophages triggered by sulforaphane via Nrf2/p38 signaling pathways

Figure 1

(a) Mabs induces Nrf2 protein expression level in THP-1-derived macrophages. Protein expression levels were normalized to β-actin in the total protein extracts, and to Lamin A/C in the nuclear extracts. (b) RT-qPCR: Nrf2 signaling pathway is activated by M. abscessus and SFN. The Nrf2 targets HO-1 and NQO1 mRNA expression levels were normalized to the housekeeping gene ubiquitin. Data shown are the means±S.E.M. of three independent experiments done in triplicates. (c) Protein expression levels of HO-1 and NQO1 were detected by immunoblotting. Densitometric quantification of protein signals was normalized to β-actin.

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