Figure 2 | Cell Death Discovery

Figure 2

From: Caspase-independent apoptosis in infected macrophages triggered by sulforaphane via Nrf2/p38 signaling pathways

Figure 2

SFN showed a decrease in mycobacterial burden in infected macrophages. THP-1-derived macrophages were pretreated with DMSO or 10 μM SFN 3 h before infection with M. abscessus for 3 h. The unincorporated mycobacteria were incubated in culture medium containing 250 μg/ml amikacin for 1 h before thorough washes. The cells were then incubated in medium with 50 μg/ml amikacin for the indicated time period (0, 1, 3, and 7 days). Intracellular mycobacteria were released by cell lysing in ice-cold water, serially diluted, and seeded on agar plates. Colony-forming units were counted 5 days after incubation at 37 °C. The graph represents the means±S.E.M. of two independent experiments done in triplicates. *P<0.03, **P<0.01.

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