Figure 5 | Cell Death Discovery

Figure 5

From: Caspase-independent apoptosis in infected macrophages triggered by sulforaphane via Nrf2/p38 signaling pathways

Figure 5

Apoptosis induction by SFN in M. abscessus-infected macrophages. THP-1-derived macrophages pretreated with SFN or DMSO for 3 h were infected with M. abscessus at the MOI of 10:1 for 24 or 48 h. (a) Annexin V-FITC (green) and Mabs-mCherry (red; white arrow) were excited by 488 and 568 nm lasers, respectively, and each individual macrophage was imaged by the 40× objective of the ImageStream MARKII cytometer and data analysis was performed (n=10 000 cells). (b) The percentage of apoptotic cells was determined after the indicated post infection times using Annexin V-FITC labeling. #P<0.03 compared with SFN-treated cells. (c) Colocalization of Mabs-mCherry in annexin V-FITC positive cells. Data represent the means±S.E.M. of three independent experiments. *P<0.04 compared with Mabs-mCherry infected cells.

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