Figure 6 | Cell Death Discovery

Figure 6

From: Caspase-independent apoptosis in infected macrophages triggered by sulforaphane via Nrf2/p38 signaling pathways

Figure 6

Macrophage apoptosis induction by SFN is caspase 3/7 independent. THP-1-derived macrophages pretreated with SFN or DMSO were pretreated for 3 h before mycobacterial infection. Caspase 3/7 activities were determined using FLICA inhibitor probe. (a) FLICA inhibitor probe (green) and Mabs-mCherry (red indicated by white arrows) were excited by a 488-nm laser and a 568-nm laser, respectively. Each individual macrophage was imaged by the 40× objective of the ImageStream MARKII cytometer and data analysis was performed on n=10 000 events. (b) Results were expressed as the percentage of FLICA-positive cells. *P<0.04 compared with DMSO-treated cells, and #P<0.001 compared with SFN-treated cells. (c) Colocalization of Mabs-mCherry in FLICA-positive cells. Data represent the means±S.E.M. of three independent experiments.

Back to article page