Figure 7
Nrf2 signaling pathway regulates MAPK cascade. (a) THP-1-derived macrophages were pretreated with MAPK-specific inhibitors PD98059, SB203580, and SP600125 of ERK, p38, and JNK, respectively, 2 h before SFN stimulation and/or Mabs infection. Cells were lysed 24 h after infection and protein lysates were analyzed by western blot. (b) THP-1-derived macrophages were transfected with scrambled or Nrf2 siRNA 24 h before SFN stimulation and/or M. abscessus infection. Cells were incubated for an additional 24 h after infection before lysis and western blots were performed using Nrf2 antibody and GAPDH antibody, as an internal control. (c) Immunoblots were performed on total protein lysates using specific phosphorylated antibodies against ERK, JNK, and p38.
