Figure 1 | Cell Death Discovery

Figure 1

From: The p53 co-activator Zac1 neither induces cell cycle arrest nor apoptosis in chicken Lim1 horizontal progenitor cells

Figure 1

Overexpression of mZac1 in the embryonic chicken retina. Quantitative RT-PCR and immunohistochemistry was used to analyse mRNA and protein expression. (a) Relative mZac1 mRNA levels 24 and 48 h after electroporation of mZac1 or control vectors of st22 retinas. (b) Relative mRNA levels of mZac1 and GFP 24 and 48 h after electroporation. (c and d) Fluorescence micrographs of retinas electroporated with mZac1 at st22 and analysed after 24 and 48 h showing mZac1 and GFP IR. Dashed line marks border to mZac1 electroporated region. (e and f) Fluorescence micrographs of retinas electroporated with the control vector at st22 and analysed after 24 or 48 h. Control: GFP vector, gcl: ganglion cell layer (basal side). Student’s t-test, *P<0.05, **P<0.01, ***P<0.001, N≥4, mean±S.D.

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