Figure 3 | Cell Death Discovery

Figure 3

From: The p53 co-activator Zac1 neither induces cell cycle arrest nor apoptosis in chicken Lim1 horizontal progenitor cells

Figure 3

Overexpression of mZac1 induced apoptosis. Apoptosis was analysed by immunohistochemical detection of C-Casp-3+ and TUNEL in mZac1 or control electroporated retinas. C-casp-3+ IR cells were counted. (a) Relative density of C-Casp-3+ cells in retinas counted at 24, 48 and 72 h after electroporation. (b and c) Fluorescence micrographs showing mZac1 and C-Casp-3 IR in mZac1 electroporated st22 retinas, and GFP and C-Casp3 IR in control electroporated st22 retinas, analysed after 72 h. (d) mZac1 IR and TUNEL staining in retinas electroporated at st22 and analysed after 72 h. Arrow heads indicate double-positive cells. Control: GFP vector, gcl: ganglion cell layer. Student’s t-test, *P<0.05, ***P<0.001, N≥4, mean±S.D.

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