Figure 2
From: Cytotoxic activity of the MK2 inhibitor CMPD1 in glioblastoma cells is independent of MK2
CMPD1 induces G2/M arrest in glioblastoma cells. (a) U87 cells were treated with CMPD1 or MK2i (48 h) and cell-cycle distribution was analysed by flow cytometry. Proportion of cells in each cell-cycle phase is expressed as mean±S.E.M. from three independent experiments; cells with DNA content >4n were excluded from the analysis. (b) U87 cells were treated with CMPD1 (5 μM) for indicated time and cell-cycle distribution was analysed by flow cytometry. Bars represent proportion of cells in each cell-cycle phase; data are expressed as mean±S.E.M. from three independent experiments. (c) A representative FACS plot for U87 cells treated with CMPD1 (5 μM) for indicated time demonstrating changes in 2n, 4n, and 8n populations. (d) U87 cells were treated with CMPD1 for indicated time and cell lysates analysed with western blotting using indicated antibodies. Representative images of three independent experiments are shown.
