Figure 3
From: Polyphenols act synergistically with doxorubicin and etoposide in leukaemia cell lines
The effect of doxorubicin (DOX) and etoposide (ETP) when used in combination with quercetin (QUE), apigenin (AP), emodin (EMO), rhein (RH) or cis-stilbene (CIS) on caspase 3 activity of two lymphoid leukaemia cell lines (Jurkat and CCRF-CEM), two myeloid leukaemia cell lines (THP-1 and KG-1a) and two non-tumour control cells (CD133+ HSC and CD34+ HSC). This was evaluated by NucView caspase 3 activity assay. Cells were treated with doxorubicin or etoposide and polyphenols alone and in combination for 24 h using their lowest-significant doses (LSD), together with a vehicle control. All data were normalised to the vehicle control, which was assigned a 0% apoptotic level. The data were expressed as medians with ranges (n=4). The black bars show the vehicle controls and treatments alone; the coloured bars indicate significant additive effects in brown, synergistic effects in yellow, competitive antagonistic effects in purple and antagonistic effects in pink. Statistical significance was set at P⩽0.05 compared with vehicle control, drugs alone and expected values of individual drugs effects.
